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Times and temperatures used for the PCR configuration with SYBR Green I and the LC
| Parameter | Temp (°C) | Time (s) | Slope (°C/s) | Acquisition mode |
|---|---|---|---|---|
| Denaturation | 95 | 8 | 20 | None |
| Amplification (40 cycles) | 95 | 10 | 20 | None |
| 56 | 10 | 20 | None | |
| 72 | 8 | 20 | Single | |
| Melting | 95 | 10 | 20 | None |
| 67 | 30 | 20 | None | |
| 95 | 10 | 0.1 | Continue | |
| Cooling | 40 | 60 | 20 | None |
