TABLE 1.

 Influence of VLP adsorption temperature on ELISA results^a

HPV16 DNA status of the sample	No. of samples	25°C			4°C
		No. (%) of samples with OD of 0.16	Mean OD	CV (%)	No. (%) of samples with OD of 0.16	Mean OD	CV (%)
Positiveb	11	9 (82)	0.581	25.6	9 (82)	0.703	2.8
Negativec	29	11 (38)	0.188	45.1	2 (7)	0.065	8.7

^aSerum samples were from women infected with known cervical HPV DNA types and with known disease status (24). OD and CV values between VLP adsorption at 25 and 4°C were compared by the paired t test. The difference in the proportion of seropositive samples between the two conditions among the HPV DNA-negative samples was evaluated by the McNemar test.\

^bAmong the HPV16 DNA-positive samples, comparison of VLP adsorption at 25 and 4°C for differences in ODs, P = 0.206; comparison for differences in CVs, P = 0.014.\

^cAmong the HPV16 DNA-negative samples, comparison of VLP adsorption at 25 and 4°C for differences in ODs, P ≤ 0.001; comparison for differences in CVs, P < 0.001; comparison for differences in the proportions of samples with false-positive results, P = 0.004.