TABLE 1.
HPV16 DNA status of the sample | No. of samples | 25°C
|
4°C
|
||||
---|---|---|---|---|---|---|---|
No. (%) of samples with OD of 0.16 | Mean OD | CV (%) | No. (%) of samples with OD of 0.16 | Mean OD | CV (%) | ||
Positiveb | 11 | 9 (82) | 0.581 | 25.6 | 9 (82) | 0.703 | 2.8 |
Negativec | 29 | 11 (38) | 0.188 | 45.1 | 2 (7) | 0.065 | 8.7 |
Serum samples were from women infected with known cervical HPV DNA types and with known disease status (24). OD and CV values between VLP adsorption at 25 and 4°C were compared by the paired t test. The difference in the proportion of seropositive samples between the two conditions among the HPV DNA-negative samples was evaluated by the McNemar test.\
Among the HPV16 DNA-positive samples, comparison of VLP adsorption at 25 and 4°C for differences in ODs, P = 0.206; comparison for differences in CVs, P = 0.014.\
Among the HPV16 DNA-negative samples, comparison of VLP adsorption at 25 and 4°C for differences in ODs, P ≤ 0.001; comparison for differences in CVs, P < 0.001; comparison for differences in the proportions of samples with false-positive results, P = 0.004.