TABLE 4.
Influence of PVP-360 on assays with samples from women positive for cervical HPV16 DNAa
| Reagent | Mean OD
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Pool 1 | Pool 2 | Pool 3 | Pool 4 | Pool 5 | Pool 6 | Pool 7 | Pool 8 | Pool 9 | Pool 10 | |
| Milk | 0.134 | 0.974 | 0.224 | 1.562 | 0.938 | 1.17 | 0.953 | 0.522 | 0.042 | 0.006 |
| Milk + PVP-360 | 0.232 | 1.090 | 0.376 | 1.538 | 1.131 | 1.315 | 1.153 | 0.764 | 0.087 | 0.019 |
| PVA-50 + Tween 20 | 0.576 | 1.188 | 0.449 | 1.688 | 1.222 | 1.489 | 1.202 | 0.827 | 0.144 | 0.07 |
| PVA-50 + Tween 20 + PVP-360 | 0.785 | 1.281 | 0.676 | 1.564 | 1.264 | 1.444 | 1.267 | 1.010 | 0.275 | 0.165 |
a
The compositions of the serum pools are described in Materials and Methods. A two-way analysis of variance was used to assess the statistical significance between the average ODs obtained with different blocking solutions (P < 0.0001). Milk, PVP-360, Tween 20, and PVA-50 were added to the blocking solution at final concentrations of 5, 0.8, 0.05, and 0.5%, respectively. Values represent the mean OD for each serum pool run twice.