TABLE 5.
Influence of PVP-360 on assays with control samplesa
Reagent | Mean OD
|
|||||||||
---|---|---|---|---|---|---|---|---|---|---|
Control 1 | Control 2 | Control 3 | Control 4 | Control 5 | Control 6 | Control 7 | Control 8 | Control 9 | Control 10 | |
Milk | 0.008 | 0.000 | 0.018 | 0.004 | 0.002 | 0.004 | 0.001 | 0.002 | 0.002 | 0.000 |
Milk + PVP-360 | 0.017 | 0.010 | 0.045 | 0.003 | 0.015 | 0.001 | 0.007 | 0.003 | 0.014 | 0.011 |
PVA-50 + Tween 20 | 0.054 | 0.030 | 0.034 | 0.016 | 0.037 | 0.017 | 0.027 | 0.043 | 0.046 | 0.028 |
PVA-50 + Tween 20 + PVP-360 | 0.074 | 0.037 | 0.043 | 0.023 | 0.053 | 0.032 | 0.043 | 0.057 | 0.059 | 0.063 |
Control samples were obtained from HPV16 DNA-negative virginal women (serum set 1). PVP-360 was added to the conjugate buffers at a final concentration of 0.8%, Tween 20 was added at a final concentration of 0.05%, milk was added at a final concentration of 5%, and PVA-50 was added at a final concentration of 0.5%. Values represent the mean OD of each sample run twice.