TABLE 4.

 Bacteria detected in carious dentine by real-time PCR

Bacterium	No. of cells mg of dentine−1a
	Range	Median	Mean ± SEMc
Total anaerobicb	2.1 × 107-1.1 × 109	2.6 × 108	(3.0 ± 0.3) × 108
Prevotella	0.0-(2.0 × 108)	1.6 × 107	(3.9 ± 0.7) × 107
P. melaninogenica	0.0-(3.5 × 106)	7.6 × 104	(2.6 ± 0.7) × 105
Fusobacterium	4.8 × 102-5.7 × 106	7.2 × 104	(4.2 ± 1.1) × 105
M. micros	0.0-(1.6 × 107)	3.6 × 105	(1.5 ± 0.5) × 106
P. endodontalis	0.0-(1.3 × 106)	6.8 × 103	(8.4 ± 3.7) × 104
P. gingivalis	0.0-(2.5 × 104)	1.0 × 103	(3.9 ± 1.0) × 103

^aData collected for 65 samples.\

^bDetected with the universal primer and probe set (23).\

^cDetermined from triplicate measurements.