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Plasmid construction of human SF-1 LBD. SF-1 LBD (218-461) plasmid design uses a pET-based backbone with a standard T7 promoter for IPTG induction, with an N-termal 6X-His tag and a Tev cleavage site. Table below explains the function of each element contained in the vector, image from Vector Bee, table adapted from information provided by VectorBuilder.
