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Chromatography Isolation of SF-1: PIP3. Anion Exchange Chromatography was used for collection of PIP3 bound SF1-LBD by MonoQ 5/50 Cytivia Column. Featured in this figure is a chromatogram of percentage of Buffer B needed to elute off SF-1:PIP3. Protein elution is identified by absorbance values (mAU)
