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. 2026 Apr 14;123(16):e2523148123. doi: 10.1073/pnas.2523148123

Fig. 1.

A ten-panel figure shows Trem2 expression in human and mouse brain with graphs and plots for different conditions and cell types.

Trem2 expression in stroke patients and animal models. (A and B) Trem2 mRNA expression [–log2(counts + 1) normalized] in brains of stroke patients during the acute–subacute (n = 6) and chronic phases (n = 5), based on GEO datasets GSE162955 and GSE56267. (C–E) Trem2 expression in a scRNA-seq dataset using mouse MCAO model (GSE142445). Uniform Manifold Approximation and Projection plots show cell classifications in MCAO and sham mice (C), with no increase in microglial proportion after MCAO. Trem2 is primarily expressed in microglia, with similar cell subpopulation distributions across sham, contralateral (Contra), or ipsilateral (Ipsi) samples at different time points (D), and no significant changes in microglial Trem2 levels (E). (F) Experimental scheme of the photothrombotic (PT) stroke model. (G–I) Trem2 mRNA (qPCR) (G), protein levels (western blot) (H), and immunostaining (I) at 7 days post-injury (DPI) in contralateral (Contra), infarction core (Core), and peri-infarction (Peri) regions (n = 3), normalized to wild-type (WT) contralateral controls. (J) Plasma sTrem2 levels in PT and sham mice at 7 and 42 DPI measured by the enzyme-linked immunosorbent assay (ELISA) (n = 5).