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. 2026 Apr 14;123(16):e2523148123. doi: 10.1073/pnas.2523148123

Fig. 3.

A six-panel figure shows a timeline, brain scans, and graphs of neurological function, cell density, and cytokine levels in Trem 2 WT mice injected with sTrem2 or PBS.

sTrem2 aggravates ischemic injury and poststroke neuroinflammation. (A) Experimental scheme of recombinant sTrem2 or PBS injection before PT-induced stroke, followed by assessments of neurological function, infarction size, neuronal survival, microglial proliferation, and cytokine expression. (B) Neurological function was evaluated by mNSS at 7 DPI in sTrem2- and PBS-treated mice (n = 6). (C and D) Nissl staining and NeuN immunostaining at 7 DPI were used to quantify infarction volume and peri-infarction neuronal survival in the two groups (C: n = 13; D: n = 5). (E) Iba1 and EdU costaining was performed to quantify total and proliferating microglia, as well as the proportion of proliferating microglia in the peri-infarction zone between groups (n = 5). (F) ELISA of IL-1β, TNFα, IL-6, and IL-10 levels in defined brain regions (n = 5).