Table 1.
Intergenic (crossover) recombination in rec12 mutants.
| Genetic interval (chromosome) | No. testeda | No. recombinanta | Recombinant freq. (%) | cMb | cM in wild-typec | Fold reductionc |
| A. rec12-D15 | ||||||
| lys1 – his1 (I) | 500 | 3 | 0.6 | 0.6 | 89 | 148 |
| lys1 – ade4 (I) | 500 | 8 | 1.6 | 1.6 | 284 | 177 |
| his1 – ade2 (I) | 500 | 2 | 0.4 | 0.4 | 57 | 142 |
| his1 – tps19 (I) | 500 | 2 | 0.4 | 0.4 | 143 | 357 |
| ade2 – tps19 (I) | 500 | 0 | ≤ 0.2 | ≤ 0.2 | 86 | ≤ 430 |
| his4 – ade1 (II) | 500 | 6 | 1.2 | 1.2 | 63 | 53 |
| ade6 – arg1 (III) | 500 | 1 | 0.2 | 0.2 | 97 | 486 |
| mean = | 256 ± 154 | |||||
| B. rec12-Y98F | ||||||
| lys1 – his1 (I) | 500 | 3 | 0.6 | 0.6 | 89 | 148 |
| lys1 – ade4 (I) | 500 | 17 | 3.4 | 3.5 | 284 | 81 |
| his1 – ade2 (I) | 500 | 1 | 0.2 | 0.2 | 57 | 285 |
| his1 – tps19 (I) | 500 | 1 | 0.2 | 0.2 | 143 | 715 |
| ade2 – tps19 (I) | 500 | 0 | ≤ 0.2 | ≤ 0.2 | 86 | ≤ 430 |
| his4 – ade1 (II) | 500 | 5 | 1.0 | 1.0 | 63 | 63 |
| ade6 – arg1 (III) | 500 | 4 | 0.8 | 0.8 | 97 | 121 |
| mean = | 263 ± 220 | |||||
aSpore colonies were replica plated on non-selective and selective media; the number of colonies recombinant for each genetic interval are indicated. Diploid (potentially complementing) spore colonies were excluded from analysis. bGenetic distance calculated using the mapping function of Haldane [66]. cFold reduction in crossing over relative to published map distances in wild-type cells (NCBI Entrez Genome) [36]. The strains crossed were: WSP1826 × WSP1967; WSP1825 × WSP1962; WSP1967 × WSP1973; WSP1967 × WSP1973; WSP1967 × WSP1973; WSP2015 × WSP2018; WSP2026 × WSP2024; WSP2020 × WSP1966; WSP1670 × WSP1968; WSP1966 × WSP2027; WSP1966 × WSP2027; WSP1966 × WSP2027; WSP2014 × WSP2011; WSP1809 × WSP2028. Strain genotypes are provided in Table 3.