Fig. 2.

Role of TRIP13 in PCa cell proliferation and invasion. (A) Immunoblot analysis showed the protein expression of TRIP13 in DU145 and PC3 PCa cells treated with either of two specific or independent TRIP13 siRNA duplexes. (B, C) Cell proliferation assay of DU145 and PC3 cells using either of two TRIP13 siRNA duplexes or a non-targeting siRNA control. (D, E) DU145 and PC3 cells were used in the Boyden chamber Matrigel invasion assay, in which TRIP13 was transiently knocked down using either of two independent TRIP13 siRNA duplexes. Non-targeted siRNA-treated cells served as controls. The invaded cells were stained, and the absorbance was read at 560 nm. The bar graphs are shown with ± SEM. (F) Boyden chamber Matrigel invasion assay showing that overexpression of P4HA1 in RWPE-1 cells elevated their invasive potential.