Fig. 3.

DCZ0415 treatment reduces cell growth and colony formation, induces apoptosis, and modulates EMT markers in PCa cells. (A) DU145 and PC3 cells were treated for 96 hr with vehicle (DMSO) or with DCZ0415 (5-20 µM), and cell viability was examined by MTT assays. Error bar indicates mean ± SD, *p < 0.01, **p < 0.001. (B) DU145 and PC3 cells were seeded in six-well plates and treated for 14 days with a vehicle or with DCZ0415 (5-20 µM). The colonies were stained with crystal violet. (C) Quantification was performed by extracting colonies with 10% acetic acid. Error bars indicate means ± SD, **P < 0.001. DU145 and PC3 cells were treated for 48 hr with vehicle (DMSO) or DCZ0415 (5-20 µM), and cell extracts were prepared for immunoblot analysis. (D) Expressions of PCNA, cleaved caspase 3 and TRIP13, and (E) Expressions of N-cadherin, E-cadherin and vimentin in DU145 and PC3 cells treated with vehicle or DCZ0415.