. 2026 Mar 12;16:13255. doi: 10.1038/s41598-026-43837-6

Table 2.

Strains.

Strain	Genotype, relevant characteristics	Source or reference
E. coli XL1-Blue	recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F’proAB lacI ^q Z∆M15 Tn10 (Tet ^R )]	⁵³
E. coli MG1655	F⁻ λ- ilvG- rfb-50 rph-1	⁵⁴
WT	Synechococcus elongatus PCC7942	Pasteur culture collection
pipY	ΔpipY::cat, Cm^R	¹
pipXY	ΔpipXpipY::cat, Cm^R	¹
pipY 1^SPtrc	ΔpipY::cat NSI::(cs3 lacI P_trc), Sm^R	This work
pipY 1^SPtrc-PipY	ΔpipY::cat NSI::(cs3 lacI P_trc:pipY), Cm^R Sm^R	¹
pipY 1^SPtrc-PipY^K26A	ΔpipY::cat NSI::( (cs3 lacI P_trc:pipY^{76aag> gcg}), Cm^R Sm^R	This work
pipY 1^SPtrc-PipY^P63L	ΔpipY::cat NSI::(cs3 lacI P_trc:pipY^{199ccc> ctg}), Cm^R Sm^R	This work
pipY 1^SPtrc-PipY^R210Q	ΔpipY::cat NSI::(cs3 lacI P_trc:pipY^{628cga> caa}), Cm^R Sm^R	This work
pipXpipY 1^SPtrc	ΔpipXpipY::cat NSI::(cs3 lacI P_trc), Cm^R Sm^R	This work
pipXpipY 1^SPtrc-PipY	ΔpipXpipY::cat NSI::(cs3 lacI P_trc:pipY), Cm^R Sm^R	¹
pipXpipY 1^SPtrc-PipY^K26A	ΔpipXpipY::cat NSI::(cs3 lacI P_trc:pipY^{76aag> gcg}), Cm^R Sm^R	This work
pipXpipY 1^SPtrc-PipY^P63L	ΔpipXpipY::cat NSI::(cs3 lacI P_trc:pipY^{199ccc> ctg}), Cm^R Sm^R	This work
pipXpipY 1^SPtrc-PipY^R210Q	ΔpipXpipY::cat NSI::(cs3 lacI P_trc:pipY^{628cga> caa}), Cm^R Sm^R	This work