TABLE 5.
Changes in relative abundance of proteins in H. volcanii other than c-type cytochromes that may be related to electron transport proteinsa
| Locus ID | Gene product | Gene name | Fold change in relative abundance [Fe(III) vs O2] | P value |
|---|---|---|---|---|
| B9LWX1 | ABC transporter | Hlac_3452 | 2.87 | 1.54 × 10−5 |
| B9LPL7 | Copper-binding protein | Hlac_1722 | 2.62 | 8.40 × 10−6 |
| B9LQL3 | Binding-protein-dependent transport systems inner membrane component | Hlac_2056 | 2.94 | 1.41 × 10−5 |
| B9LQL2 | ABC transporter | Hlac_2055 | 3.13 | 7.52 × 10−6 |
| B9LT09 | Molybdate/tungstate import ATP-binding protein WtpC | Hlac_0472 | 1.64 | 6.05 × 10−3 |
| B9LUX4 | ABC transporter | Hlac_0852 | 2.71 | 9.57 × 10−5 |
| B9LU73 | Binding-protein-dependent transport systems inner membrane component | Hlac_2696 | 2.63 | 1.45 × 10−6 |
| A0A841HA16 | V-type ATP synthase subunit I | HNR49_000637 | 1.71 | 4.71 × 10−4 |
| B9LS41 | V-type ATP synthase alpha chain | atpA | 1.62 | 5.08 × 10−3 |
| A0A841HBA9 | Aconitate hydratase | HNR49_000801 | 1.61 | 5.49 × 10−3 |
| Q59455 | Pyruvate ferredoxin oxidoreductase alpha subunit (Fragment) | HNR49_001572 | 1.88 | 3.81 × 10−8 |
| B9LRY7 | Acetamidase/formamidase | Hlac_2285 | 2.47 | 4.13 × 10−3 |
| B9LMJ9 | Acetate-CoA ligase | Hlac_0990 | 1.77 | 3.06 × 10−4 |
| B9LN38 | Methylmalonyl-CoA mutase, large subunit | Hlac_1182 | 1.74 | 0.0317 |
| B9LV71 | Aconitate hydratase 1 | Hlac_3040 | 2.05 | 9.04 × 10−4 |
| B9LNG0 | Acetate-CoA ligase | Hlac_1306 | 1.95 | 3.50 × 10−5 |
| B9LQT7 | DoxX family protein | Hlac_0083 | 1.60 | 1.09 × 10−5 |
| A0A841HAQ4 | Glutamine synthetase | HNR49_000593 | 1.80 | 0.0395 |
| A0A841HAH8 | NADH-quinone oxidoreductase subunit C/D | HNR49_001350 | 1.61 | 4.12 × 10−5 |
| B9LPU4 | Cytochrome oxidase assembly | Hlac_1803 | 3.27 | 1.15 × 10−3 |
| B9LMG3 | DoxX family protein | Hlac_0953 | 12.6 | 5.16 × 10−4 |
| B9LUZ6 | Acetolactate synthase | Hlac_0875 | 1.61 | 4.00 × 10−6 |
| B9LSM2 | GTP-dependent dephospho-CoA kinase | Hlac_2394 | 1.77 | 0.0243 |
| A0A841H7I2 | Flavin-dependent thymidylate synthase | thyX | 1.53 | 8.50 × 10−4 |
| A0A841H836 | Pyridoxal 5′-phosphate synthase subunit PdxS | pdxS | 1.50 | 0.0239 |
| B9LR55 | Glutamyl-tRNA reductase | hemA | 3.79 | 3.86 × 10−5 |
| A0A841HB82 | Quinol-cytochrome oxidoreductase complex cytochrome b subunit | HNR49_001302 | 1.74 | 2.95 × 10−4 |
| A0A841HBL7 | Quinol-cytochrome oxidoreductase complex cytochrome b subunit | HNR49_001303 | 2.20 | 0.0233 |
| B9LT38 | 4Fe-4S ferredoxin-type domain-containing protein | Hlac_0501 | 1.67 | 1.57 × 10−4 |
| Q59455 | Pyruvate ferredoxin oxidoreductase alpha subunit | HNR49_001572 | 1.88 | 3.81 × 10−8 |
| A0A841HAT9 | Fe-S cluster assembly ATP-binding protein | HNR49_001256 | 1.75 | 0.0137 |
| B9LUN1 | Cytochrome P450 | Hlac_2727 | 1.51 | 1.38 × 10−3 |
| B9LRJ2 | FAD-linked oxidase domain protein | Hlac_0210 | 1.92 | 8.03 × 10−6 |
| A0A841H9N0 | NhaP-type Na+/H+ or K+/H+ antiporter | HNR49_000572 | 1.72 | 1.26 × 10−4 |
a
Shown are genes encoding electron transport proteins that are not c-type cytochromes and whose protein abundances differed significantly between H. volcanii cells grown with lactate as the electron donor under Fe(III)-oxide-reducing conditions and those grown under O2-respiring conditions. Fold change was calculated relative to the O2 condition [Fe(III)/O2]. Proteins were considered significantly different when P ≤ 0.05.