Figure 8.

The human TDH pseudogene is expressed. (A) Clones from 2 individuals were sequenced and mapped to the TDH gene. Clone 1 skipped exon 6, and the resulting frame-shift generated a premature stop codon in exon 7 (TGA). Clone 2 skipped exon 4 and utilised a cryptic splice site in exon 6, and the resulting frame-shift generated a premature stop codon in exon 6. (B) The translation of clone 1 (exon 6 skipped) encoded a truncated 230 residue ORF. The skipped exon 4 in clone 2 does not alter the reading frame. However, the use of a cryptic splice site in exon 6, results in a premature stop codon in exon 6. Stop codons are indicated by *.