Table 1.
Isotopolog compositions of nucleosides from S. frugiperda cells grown with a mixture of [U-13C6]glucose and natural abundance glucose.
| Compound | Position | 13C NMR Chemical Shift (δ, ppm) | Coupling Constants JCC (Hz) | %13Ca | %13C13Cb |
| Cytidine (1) | 2 | 159.7 | 1.1 | ||
| 4 | 168.4 | 55.5 (5) | 1.3 | 7.3 (5) | |
| 5 | 98.9 | 55.3 (4) | 1.2 | 8.5 (4) | |
| 6 | 144.4 | 1.2 | |||
| 1' | 92.2 | 42.7 (2') | 1.6 | 22.1 (2') | |
| 2' | 76.6 | 42.5 (1') | 1.6 | 22.8 (1') | |
| 3' | 71.9 | 38.7 (4') | 1.5 | 25.3 (4') | |
| 4' | 86.4 | 38.7 (3'), 42.0 (5') | 1.6 | 23.7 (3', 5'), 2.8 (5') | |
| 5' | 63.5 | 42.0 (4') | 1.5 | 27.4 (4') | |
| Adenosine (2) | 2 | 154.7 | 1.1 | ||
| 4 | 150.6 | n.d. | |||
| 5 | 121.3 | n.d. | |||
| 6 | 157.8 | 2.7 | |||
| 8 | 142.7 | 1.1 | |||
| 1' | 90.4 | 42.7 (2') | 1.7 | 23.5 (2') | |
| 2' | 75.8 | 42.5 (1') | 1.8 | 22.8 (1') | |
| 3' | 72.7 | 38.5 (4') | 1.7 | 26.0 (4') | |
| 4' | 87.9 | 41.8 (5'), 38.3 (3') | 1.6 | 24.6 (3', 5'), 2.9 (5') | |
| 5' | 63.6 | 41.3 (4') | 1.7 | 25.9 (4') | |
| Guanosine (3) | 6 | 156.4 | 1.3 | ||
| 2 | 153.8 | 1.1 | |||
| 4 | 150.1 | 1.1 | |||
| 5 | 115.7 | 1.0 | |||
| 8 | 135.6 | 1.2 | |||
| 1' | 86.5 | 42.9 (2') | 1.7 | 22.8 (2') | |
| 2' | 73.7 | 42.9 (1') | 1.7 | 22.3 (1') | |
| 3' | 70.2 | 38.4 (4') | 1.7 | 24.1 (4') | |
| 4' | 85.2 | 41.8 (5'), 38.0 (3') | 1.6 | 26.0 (3', 5'), 2.8 (5') | |
| 5' | 61.3 | 42.0 (4') | 1.7 | 26.5 (4') | |
aabsolute 13C abundance.
brelative fraction of satellite pairs in the global 13C NMR intensity of the index carbon atom. The coupling partners of the index carbon atom are indicated in parentheses.