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Time course of changes in dye distribution of FM4-64-loaded P. meyeri pollen tubes treated with 5 μg mL−1 BFA. A to F, Confocal fluorescence images of a BFA-treated pollen tube at different times, showing BFA treatment did not inhibit the internalization of FM4-64 dye through endocytosis but the dispersed FM4-64 distributing pattern indicating BFA inhibited secretory vesicle accumulation at tube tip. Bar = 25 μm.
