Figure 7.

FM4-64 internalization in control and inhibitor-treated pollen tubes showing that dye uptake is stimulated by BFA (5 μg mL⁻¹) inhibited by latrunculin B (1 μm) and sodium azide (500 μm). Cell-associated FM4-64 fluorescence was quantified after a 12-min internalization of the dye and normalized to control pollen tubes labeling. Data shown are means ± sd and are representative of three experiments, each containing three individual measurements. CK, Control; LTB, latrunculin B; SAD, sodium azide.