Figure 5.

Chlamydomonas FKB12 functionally complements a yeast FKBP12 mutant. Wild-type JK9-3da cells were transformed with empty vector (wt). The fpr1 mutant strain lacking the FKBP12 protein was transformed with empty vector (fpr1) or with plasmids expressing wild-type Chlamydomonas FKB12 or M52V E54Q and M52K G53Q mutants. Cultures were normalized, subjected to 10-fold serial dilutions, and spotted onto SD or SGal-Leu plates without rapamycin or containing 200 nm rapamycin. Plates were incubated at 30°C for 2 or 5 d.