Figure 7.

Expression of mutant FKB12s in Chlamydomonas partially increases the sensitivity to rapamycin. A, Rapamycin sensitivity of wild-type and rap2 cells compared to rap2 cells expressing wild-type or mutant FKB12s. Serial dilutions were spotted onto TAP plates supplemented or not with 500 nm rapamycin, and incubated at 25°C under continuous illumination. Growth was recorded after 4 or 8 d. B, Levels of FKB12 protein detected by western blot. About 15 μg of total protein obtained from wild-type (wt) or rap2 mutant cells transformed with the indicated constructs were resolved in 15% polyacrylamide gels, blotted, and incubated with anti-FKB12 antibodies.