Figure 6.

Further enhanced expression of IFS genes by lactofen was seen in wounded and WGE elicitor-treated tissues. Water, lactofen (300 μm), or lactofen (300 μm) with 30 μg/mL WGE was applied to a small cut area on the abaxial surface of soybean cotyledons. In this protocol, the treated and immediately adjacent cells were harvested as proximal cells, and a transverse section 4 mm away from the edge of the wounded surface was harvested as the distal cell population. Details are described in “Materials and Methods.” RNA extraction and northern blots were as described in Figure 3A. The probe was the same IFS probe used in Figure 5.