Table II.
Transcript levelsa of the five Arabidopsis AOX genes in wild-type and transformed plant leaves
| Lineb | AOX1a | AOX1b | AOX1c | AOX1d | AOX2c |
|---|---|---|---|---|---|
| WT | 20.67a (0.04) | 34.93 (0.42) | 33.81 (0.78) | 32.64 (0.15) | >46/n.d. |
| pBI | 20.33 (0.15) | 32.41 (0.16) | 32.71 (0.22) | 30.65 (0.16) | 35.52/>46 |
| XX-1 | 16.19 (0.01) | 33.42 (0.05) | 33.33 (0.21) | 30.93 (0.20) | 35.97/>46 |
| XX-2 | 16.93 (0.08) | 32.61 (0.10) | 33.36 (0.40) | 31.45 (0.13) | >46/>46 |
| E-14 | 16.86 (0.11) | 33.67 (1.22) | 33.19 (0.50) | 30.91 (0.82) | 36.00 (0.07) |
| AS-11 | 26.51d (0.07) | 33.77 (0.07) | 33.32 (0.62) | 31.47 (0.24) | >46/34.84 |
| AS-12 | 23.50d (0.05) | 33.49 (0.46) | 33.03 (0.32) | 30.56 (0.09) | 35.95 (0.15) |
The average crossing-point value determined from duplicate LightCycler reactions. Half the range of the measurements is given in parentheses.
Wild type is untransformed Col-0; pBI is a vector-transformed control; XX-1 and X-2 are wild-type AOX overexpressors; E-14 is a mutated AOX overexpressor; AS-11 and AS-12 are anti-sense lines.
Values for the individual reactions are given, with two exceptions where the average is given; n.d., not detectable.
Crossing points for the anti-sense lines were determined from cDNA synthesized with an AOX sense transcript-specific primer. Crossing-point averages from wild type and XX-1, using the sense-specific primer, were 22.36 (0.00) and 17.96 (0.05), respectively.