Table III.
Leaf respiration characteristics of wild-type Col-0 and transformed Arabidopsis
Where dashes appear, the plant line was not included in a planting (% Total Rates) or the value given in the column refers to the group as a whole (Estimated Group Mean).
Groupb
|
Lineb
|
% Total Rate in the Presence of KCNa
|
Estimated Group Meanc
|
||
---|---|---|---|---|---|
Planting 1 | Planting 2 | Planting 3 | |||
0 | WT | 89 | 108 | 59 | 76 |
V2 | – | 77 | – | – | |
pBI | 95 | – | 48 | – | |
1 | XX-1 | – | 147 | – | 169 |
XX-2 | 331 | – | 151 | – | |
X-6 | – | 127 | – | – | |
X-3 | – | 166 | – | – | |
2 | E-4 | – | 112 | – | 130 |
E-9 | 192 | – | 108 | – | |
E-14 | – | 117 | – | – | |
3 | AS-11 | 22 | – | 23 | 29 |
AS-12 | 24 | – | 39 | – | |
ASE-10 | – | – | 26 | – |
Two replicate measurements for each line at each planting were made of the uninhibited rate and the subsequent rate established following addition of KCN (10 mm for planting 1, 2.5 mm for plantings 2 and 3). Residual O2 uptake rates, in the presence of KCN and SHAM, were subtracted from the initial (total) rate and the rate in the presence of KCN to obtain corrected rates. Percent of total rate is the ratio of the corrected +KCN rate to corrected initial rate.
Group 0, Controls (Col-0 and the vector-transformed controls, V2 and pBI); group 1, wild-type AOX overexpressors (XX-1 to X-3); group 2, mutated AOX overexpressors (E-4 to E-14); group 3, anti-sense (AS-11 and AS-12) and cosuppressed (in leaves; ASE-10) lines.
Estimated group means are inverse-transformed values of estimates from a mixed-model ANOVA using log2-transformed data.