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TE viability visualized by simultaneous calcofluor and FDA staining. Cells were cultured for 72 (A–C) and 78 h (D–F) and visualized under bright-field conditions (A and D), UV light (B and E), and blue light (C and F). TEs are characterized by calcofluor-positive blue SCW under UV light (B and E) and viable cells are stained green (FDA) under blue light (C and F). Magnification bars = 8 μm.
