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. 2005 Dec;139(4):1853–1869. doi: 10.1104/pp.105.067314

Table II.

Identification of embryo sac genes

Genes were selected on the basis of the P value of <0.0050, fold change of >2.0, and signal intensity of >60.0 at wild-type siblings. WT1–3, Mean of signal intensities for arrays 1 to 3 of wild-type siblings. spl1–3, Mean of signal intensities for arrays 1 to 3 of spl mutants. WTd1–d3, Mean of signal intensities for arrays 1 to 3 of emasculated wild-type siblings. spl1–3, d1–d2, Mean of signal intensities for arrays 1 to 3 of nonemasculated spl, and arrays 1 and 2 of emasculated spl.

Affymetrix Codea
Gene IDb
Description
Early Staged Ovule
Late Staged Ovule
Divisione
WT1–3 spl1–3 FCc P Valued WTd1–d3 spl1–3, d1–d2 FCc P Valued
Unknown protein
    261271_at At1g26795 Self-incompatibility protein related 769 30 25.3 0.000083 761 27 28.2 0.000000 Both
    265133_s_at At1g51250 Expressed protein 151 25 6.1 0.001456 539 19 28.2 0.000000 Both
    259726_at At1g60985 Expressed protein 98 16 5.9 0.002140 349 16 21.4 0.000003 Both
    265762_at At2g01240 Reticulon family protein (RTNLB15) 227 15 15.1 0.000418 148 14 10.3 0.000020 Both
    263713_at At2g20595 Expressed protein 278 15 18.9 0.003556 1,047 25 41.4 0.000000 Both
    267193_at At2g30900 Expressed protein 97 23 4.3 0.000554 108 20 5.4 0.000016 Both
    256719_at At2g34130 CACTA-like transposase family (Ptta/En/Spm) 117 11 10.3 0.000181 114 10 11.8 0.000000 Both
    256600_at At3g14850 Expressed protein 261 34 7.6 0.000088 294 35 8.5 0.000000 Both
    257889_at At3g17080 Self-incompatibility protein related 187 30 6.2 0.002931 342 19 17.8 0.000000 Both
    251698_at At3g56610 Expressed protein 518 16 32.0 0.004682 1,444 31 46.8 0.000000 Both
    254257_s_at At4g23350 Expressed protein 104 30 3.5 0.003879 335 22 15.3 0.000000 Both
    253164_at At4g35725 Expressed protein 158 15 10.4 0.003044 298 14 21.2 0.000024 Both
    250871_at At5g03930 Expressed protein 171 24 7.0 0.001971 106 14 7.5 0.000002 Both
    250325_s_at At5g12060 Self-incompatibility protein related 138 41 3.4 0.001255 206 30 6.8 0.000001 Both
    249855_at At5g22970 Expressed protein 261 25 10.6 0.003517 1,028 32 31.7 0.000001 Both
    249757_at At5g24316 Pro-rich family protein 471 17 27.7 0.001766 1,328 29 45.6 0.000000 Both
    249401_at At5g40260 Nodulin MtN3 family protein 566 37 15.2 0.000603 427 30 14.3 0.000004 Both
    248284_at At5g52975 Expressed protein 755 81 9.3 0.000353 403 49 8.2 0.000001 Both
    262503_at At1g21670 Expressed protein 161 41 4.0 0.000009 64 45 1.4 0.031616 Early
    261731_s_at At1g47780 Acyl-protein thioesterase related 84 15 5.6 0.004140 60 13 4.7 0.000003 Early
    249375_at At5g40730 Arabinogalactan protein (AGP24) 806 129 6.2 0.000342 661 575 1.1 0.245297 Early
    264610_at At1g04645 Self-incompatibility protein related 106 18 5.7 0.157950 793 21 38.1 0.000557 Late
    261846_at At1g11540 Expressed protein 145 107 1.4 0.117890 170 85 2.0 0.000012 Late
    255908_s_at At1g18010 Expressed protein 128 64 2.0 0.018058 93 45 2.1 0.000005 Late
    256079_at At1g20680 Expressed protein 56 14 3.9 0.011798 67 12 5.7 0.000019 Late
    263027_at At1g24010 Expressed protein 23 12 1.9 0.256580 96 22 4.3 0.000001 Late
    260942_s_at At1g45190 Expressed protein 318 6 49.3 0.007497 1,404 30 47.1 0.000000 Late
    265138_at At1g51300 Acyl-protein thioesterase related 192 43 4.4 0.015275 450 49 9.1 0.000000 Late
    262314_at At1g70810 C2 domain-containing protein 23 17 1.4 0.182775 65 17 3.8 0.000004 Late
    262972_at At1g75620 Glyoxal oxidase related 67 49 1.4 0.205253 133 38 3.5 0.000079 Late
    264297_at At1g78710 Expressed protein 120 62 1.9 0.005001 144 57 2.5 0.000001 Late
    267241_at At2g02490 Hydroxyproline-rich glycoprotein family protein 45 4 10.6 0.052466 395 11 37.0 0.000186 Late
    267218_at At2g02515 Expressed protein 163 39 4.2 0.007206 838 34 24.8 0.000001 Late
    265517_at At2g06090 Self-incompatibility protein related 254 14 17.8 0.013973 1,109 23 47.4 0.000000 Late
    264590_at At2g17710 Expressed protein 144 194 0.7 0.361347 841 394 2.1 0.000010 Late
    263518_at At2g21655 Expressed protein 271 17 15.8 0.015412 946 25 37.6 0.000001 Late
    265674_at At2g32190 Expressed protein 100 61 1.6 0.072973 198 70 2.8 0.000267 Late
    265670_s_at At2g32210 Expressed protein 155 158 1.0 0.888298 213 76 2.8 0.000186 Late
    265245_at At2g43060 Expressed protein 39 45 0.9 0.564913 131 62 2.1 0.000340 Late
    259107_at At3g05460 Sporozoite surface protein related 403 43 9.4 0.008348 1,415 46 31.1 0.000069 Late
    258130_at At3g24510 Expressed protein 486 10 48.6 0.012987 2,033 38 53.8 0.000000 Late
    252253_at At3g49300 Pro-rich family protein 251 42 6.0 0.007754 626 32 19.3 0.000044 Late
    251606_at At3g57840 Self-incompatibility protein related 109 39 2.8 0.058391 468 38 12.4 0.000005 Late
    255207_at At4g07515 Expressed protein 453 23 19.8 0.008275 859 32 27.0 0.000000 Late
    245424_at At4g17505 Expressed protein 157 55 2.8 0.005099 312 33 9.4 0.000003 Late
    254494_at At4g20050 Expressed protein 125 85 1.5 0.069310 230 59 3.9 0.000001 Late
    254001_at At4g26260 Expressed protein 174 62 2.8 0.067591 149 25 5.9 0.000073 Late
    253724_at At4g29285 Expressed protein 91 12 7.3 0.029036 647 16 39.5 0.000000 Late
    253656_at At4g30090 Expressed protein 101 85 1.2 0.276358 76 37 2.1 0.000137 Late
    253401_at At4g32870 Expressed protein 88 41 2.1 0.040870 165 39 4.3 0.000012 Late
    246641_s_at At5g34885 Expressed protein 216 21 10.3 0.025229 631 27 23.5 0.000000 Late
    249179_at At5g42955 Expressed protein 266 14 19.3 0.014861 882 23 38.0 0.000000 Late
    248892_at At5g46300 Expressed protein 62 5 11.8 0.014887 124 5 23.1 0.000001 Late
Central intermediary metabolism
    259786_at At1g29660 GDSL-motif lipase/hydrolase family protein 289 84 3.4 0.003166 879 425 2.1 0.000131 Both
    260124_at At1g36340 Ubiquitin-conjugating enzyme, E2 584 38 15.3 0.001661 467 34 13.8 0.000002 Both
    245672_at At1g56710 Glycoside hydrolase family 28 protein 218 85 2.6 0.004220 235 94 2.5 0.000298 Both
    257442_at At2g28680 Cupin family protein 316 47 6.8 0.001424 303 33 9.2 0.000008 Both
    267408_at At2g34890 CTP synthase, putative 152 25 6.1 0.000651 67 16 4.2 0.000011 Both
    257243_at At3g24230 Pectate lyase family protein 332 59 5.7 0.003792 198 63 3.2 0.000001 Both
    258763_s_at At3g30540 (1-4)-β-Mannan endohydrolase family 125 8 15.9 0.002254 148 9 17.4 0.000000 Both
    252342_at At3g48950 Glycoside hydrolase family 28 protein 348 30 11.6 0.001576 603 26 23.3 0.000000 Both
    248925_at At5g45910 GDSL-motif lipase/hydrolase-like protein 279 14 20.0 0.001613 1,367 26 53.5 0.000000 Both
    247228_at At5g65140 Trehalose-6-phosphate phosphatase 305 38 8.0 0.000647 246 92 2.7 0.000044 Both
    264147_at At1g02200 CER1 protein 206 85 2.4 0.000205 40 39 1.0 0.799875 Early
    264146_at At1g02205 CER1 protein, At1g02200 650 279 2.3 0.000021 219 225 1.0 0.820363 Early
    259703_at At1g77790 Glycosyl hydrolase family 17 protein 113 16 7.0 0.000428 54 11 4.8 0.000000 Early
    267202_s_at At2g31030 Oxysterol-binding family protein 74 10 7.3 0.000864 27 7 3.8 0.000005 Early
    260611_at At2g43670 Glycosyl hydrolase family 17 protein 132 53 2.5 0.002326 129 75 1.7 0.000212 Early
    252320_at At3g48580 Xyloglucan:xyloglucosyl transferase, putative 743 315 2.4 0.003795 1,176 766 1.5 0.004917 Early
    250082_at At5g17200 Glycoside hydrolase family 28 protein 71 17 4.2 0.002910 20 11 1.8 0.000578 Early
    260947_at At1g06020 PfkB-type carbohydrate kinase family protein 58 40 1.5 0.010463 75 29 2.6 0.000134 Late
    259391_s_at At1g06350 Fatty acid desaturase family protein 163 98 1.7 0.342723 196 88 2.2 0.000376 Late
    255956_at At1g22015 Galactosyltransferase family protein 158 43 3.7 0.007113 274 34 8.0 0.000000 Late
    245792_at At1g32100 Pinoresinol-lariciresinol reductase, putative 66 66 1.0 0.993453 184 68 2.7 0.000127 Late
    245794_at At1g32170 Xyloglucan:xyloglucosyl transferase, putative 59 49 1.2 0.363717 101 47 2.1 0.000150 Late
    256211_at At1g50960 Gibberellin 20-oxidase related 42 19 2.2 0.015340 75 14 5.4 0.000000 Late
    260333_at At1g70500 Polygalacturonase, putative/pectinase, putative 581 27 21.7 0.007959 674 42 16.0 0.000001 Late
    260066_at At1g73610 GDSL-motif lipase/hydrolase family protein 69 24 2.9 0.035163 163 20 8.0 0.000056 Late
    260259_at At1g74300 Esterase/lipase/thioesterase family protein 60 57 1.1 0.690243 105 47 2.2 0.000095 Late
    265331_at At2g18420 Gibberellin-responsive protein, putative 27 25 1.1 0.777953 73 18 4.1 0.000139 Late
    267607_s_at At2g26740 Epoxide hydrolase, soluble (sEH) 138 137 1.0 0.977676 205 87 2.4 0.000242 Late
    267337_at At2g39980 Transferase family protein 93 46 2.0 0.015251 69 30 2.3 0.000006 Late
    260559_at At2g43860 Polygalacturonase, putative/pectinase, putative 113 38 3.0 0.005920 69 30 2.3 0.000032 Late
    258767_at At3g10890 (1-4)-β-Mannan endohydrolase, putative 100 16 6.3 0.013753 698 20 35.1 0.000002 Late
    258151_at At3g18080 Glycosyl hydrolase family 1 protein 199 194 1.0 0.899935 384 124 3.1 0.000003 Late
    257065_at At3g18220 Phosphatidic acid phosphatase family protein 45 29 1.5 0.034020 63 22 2.8 0.000003 Late
    251491_at At3g59480 PfkB-type carbohydrate kinase family protein 549 56 9.9 0.032782 598 210 2.8 0.001882 Late
    255550_at At4g01970 Galactinol-raffinose galactosyltransferase, putative 127 41 3.1 0.006794 226 83 2.7 0.000170 Late
    245349_at At4g16690 Esterase/lipase/thioesterase family protein 75 40 1.9 0.193063 112 53 2.1 0.000368 Late
    254609_at At4g18970 GDSL-motif lipase/hydrolase family protein 461 508 0.9 0.189942 703 309 2.3 0.000085 Late
    246498_at At5g16230 Acyl-[acyl-carrier-protein] desaturase, putative 34 26 1.3 0.219929 84 23 3.6 0.001229 Late
    249983_at At5g18470 Curculin-like (mannose-binding) lectin family protein 44 35 1.2 0.486888 106 48 2.2 0.001507 Late
    246774_at At5g27530 Glycoside hydrolase family 28 protein 108 46 2.4 0.012277 146 31 4.8 0.000001 Late
    249474_s_at At5g39190 Germin-like protein (GER2) 14 12 1.2 0.500732 99 13 7.6 0.000004 Late
    248812_at At5g47330 Palmitoyl protein thioesterase family protein 63 78 0.8 0.143704 203 43 4.7 0.000004 Late
    248791_at At5g47350 Palmitoyl protein thioesterase family protein 212 65 3.3 0.040644 1,167 291 4.0 0.000003 Late
Hypothetical protein
    260318_at At1g63960 Hypothetical protein 62 20 3.2 0.002924 72 20 3.5 0.000014 Both
    265579_at At2g20070 Hypothetical protein 661 48 13.8 0.000041 487 36 13.4 0.000000 Both
    257434_at At2g21740 Hypothetical protein 128 25 5.0 0.001238 301 20 15.3 0.000000 Both
    252753_at At3g43500 Hypothetical protein 80 20 4.0 0.002002 131 20 6.6 0.000002 Both
    246859_at At5g25950 Hypothetical protein 70 13 5.4 0.000045 131 12 10.9 0.000000 Both
    248225_at At5g53740 Hypothetical protein 168 72 2.3 0.000851 111 52 2.1 0.000065 Both
    266706_at At2g03320 Hypothetical protein 97 28 3.5 0.001116 47 17 2.8 0.000175 Early
    246866_at At5g25960 Hypothetical protein 131 11 11.7 0.000000 41 9 4.8 0.000020 Early
    248396_at At5g52130 Hypothetical protein 111 21 5.4 0.000609 47 15 3.2 0.000216 Early
    257468_at At1g47470 Hypothetical protein 147 13 11.2 0.029273 1,010 25 40.1 0.000000 Late
    261313_at At1g52970 Hypothetical protein 484 49 9.9 0.007905 1,881 59 32.1 0.000000 Late
    259944_at At1g71470 Hypothetical protein 72 60 1.2 0.213121 71 33 2.1 0.000104 Late
    263895_at At2g21920 Hypothetical protein 107 26 4.2 0.007542 73 17 4.2 0.000906 Late
    258798_at At3g04540 Hypothetical protein 202 55 3.7 0.087047 1,542 50 30.9 0.000000 Late
    256773_at At3g13630 Hypothetical protein 31 28 1.1 0.715677 60 27 2.2 0.000839 Late
    251607_at At3g57850 Hypothetical protein 73 11 6.6 0.036056 370 13 29.4 0.000050 Late
    255029_x_at At4g09470 Hypothetical protein 139 8 17.2 0.012714 687 16 43.5 0.000003 Late
    255804_at At4g10220 Hypothetical protein 120 31 3.9 0.009652 410 34 12.0 0.000003 Late
    254692_at At4g17860 Hypothetical protein 60 35 1.7 0.028983 107 28 3.9 0.000008 Late
    246472_at At5g17130 Hypothetical protein 80 83 1.0 0.853233 75 34 2.2 0.000487 Late
    249157_at At5g43510 Hypothetical protein 356 13 27.3 0.012178 588 29 20.1 0.000001 Late
    247245_at At5g64720 Hypothetical protein 105 15 6.9 0.010392 322 13 24.5 0.000000 Late
Detoxification/stress response
    264001_at At2g22420 Peroxidase 17 (PER17) 791 121 6.5 0.003718 725 120 6.1 0.000007 Both
    265264_at At2g42930 Glycosyl hydrolase family protein 17 346 55 6.3 0.003492 285 34 8.5 0.000007 Both
    254000_at At4g26250 Galactinol synthase induced by water stress 89 18 4.8 0.003892 65 15 4.2 0.000004 Both
    252951_at At4g38700 Disease resistance-responsive family protein 248 109 2.3 0.004228 706 115 6.2 0.000000 Both
    247857_at At5g58400 Peroxidase, putative 1,053 240 4.4 0.000004 473 161 2.9 0.000023 Both
    261410_at At1g07610 Metallothionein-like protein 1C (MT-1C) 333 159 2.1 0.000832 238 170 1.4 0.168590 Early
    266743_at At2g02990 Ribonuclease 1 (RNS1) 67 23 2.9 0.001354 140 100 1.4 0.201557 Early
    263026_at At1g24000 Bet v I allergen family protein 43 15 2.9 0.266278 250 19 12.9 0.000001 Late
    265920_s_at At2g15120 Pseudogene, disease-resistance family protein 38 33 1.1 0.299880 103 23 4.6 0.000933 Late
    266562_at At2g23970 Defense-related protein, putative 68 60 1.1 0.479766 194 38 5.1 0.000000 Late
    267138_s_at At2g38210 Ethylene-responsive protein, putative 181 86 2.1 0.091609 246 76 3.2 0.001233 Late
    266169_at At2g38900 Ser protease inhibitor 40 37 1.1 0.867313 343 105 3.3 0.000002 Late
    260557_at At2g43610 Glycoside hydrolase family 19 protein 94 49 1.9 0.003261 76 33 2.3 0.000002 Late
    258791_at At3g04720 Hevein-like protein (HEL) 35 35 1.0 0.937369 91 34 2.6 0.003414 Late
    258172_at At3g21620 Early responsive to dehydration protein related 60 24 2.5 0.000597 68 21 3.2 0.000002 Late
    254098_at At4g25100 Superoxide dismutase (Fe), chloroplast (SODB) 688 313 2.2 0.060518 76 14 5.6 0.000220 Late
    253655_at At4g30070 Plant defensin-fusion protein, putative 99 39 2.5 0.007716 167 23 7.4 0.000001 Late
    250200_at At5g14130 Peroxidase, putative 94 67 1.4 0.229669 106 41 2.6 0.000012 Late
    250083_at At5g17220 Glutathione S-transferase-like protein 99 93 1.1 0.870900 537 235 2.3 0.000020 Late
    249560_at At5g38330 Plant defensin-fusion protein, putative 280 26 11.0 0.008435 1,140 29 39.5 0.000000 Late
    249527_at At5g38710 Pro oxidase, putative 70 18 3.9 0.006176 74 15 4.8 0.000019 Late
Cell structure organization
    260573_at At2g47280 Pectinesterase family protein 227 36 6.4 0.000454 139 25 5.5 0.000000 Both
    257878_at At3g17150 Invertase/pectin methylesterase inhibitor family 399 26 15.3 0.004375 1,121 38 29.2 0.000000 Both
    258438_at At3g17230 Invertase/pectin methylesterase inhibitor family 434 30 14.4 0.000924 758 30 24.8 0.000000 Both
    251748_at At3g55680 Invertase/pectin methylesterase inhibitor family 162 51 3.2 0.000901 112 39 2.8 0.000002 Both
    255699_at At4g00190 Putative pectinesterase 86 31 2.8 0.001537 90 24 3.7 0.000001 Both
    248823_s_at At5g46960 Invertase/pectin methylesterase inhibitor family 270 8 35.1 0.002890 519 13 39.4 0.000003 Both
    247246_at At5g64620 Invertase/pectin methylesterase inhibitor family 747 338 2.2 0.000298 870 425 2.0 0.000000 Both
    260802_at At1g78400 Glycoside hydrolase family 28 protein 201 23 8.8 0.000001 30 18 1.7 0.001391 Early
    258764_at At3g10720 Pectinesterase, putative 415 205 2.0 0.001974 112 77 1.5 0.008263 Early
    253725_at At4g29340 Profilin 3 (PRO3) (PFN3) 63 27 2.3 0.000446 26 23 1.1 0.244366 Early
    264500_at At1g09370 Pectinesterase inhibitor domain-containing protein 61 10 6.0 0.123949 594 14 43.8 0.000012 Late
    259613_at At1g48010 Invertase/pectin methylesterase inhibitor family 38 36 1.1 0.597625 105 29 3.6 0.000000 Late
    262083_at At1g56100 Pectinesterase inhibitor domain-containing protein 128 53 2.4 0.444629 2,622 746 3.5 0.000012 Late
    245656_at At1g56620 Pectinesterase inhibitor domain-containing protein 50 26 1.9 0.044275 252 19 13.3 0.000000 Late
    257679_at At3g20470 Pseudogene, Gly-rich protein 117 99 1.2 0.700308 578 267 2.2 0.001050 Late
    245371_at At4g15750 Invertase/pectin methylesterase inhibitor family 200 63 3.2 0.067454 1,378 440 3.1 0.000004 Late
    249962_at At5g18990 Pectinesterase family protein 39 19 2.0 0.028245 82 15 5.5 0.000041 Late
    247377_at At5g63180 Pectate lyase family protein 175 125 1.4 0.147262 410 192 2.1 0.000518 Late
Transport
    260319_at At1g63950 Heavy-metal-associated domain-containing protein 310 28 10.9 0.002905 148 19 7.9 0.000001 Both
    259757_at At1g77510 Protein disulfide isomerase, putative 1,163 447 2.6 0.000344 943 367 2.6 0.000170 Both
    258760_at At3g10780 Emp24/gp25L/p24 family protein 435 94 4.6 0.001770 400 75 5.4 0.000000 Both
    245892_at At5g09370 Protease inhibitor/seed storage/lipid transfer protein 384 57 6.8 0.004630 1,326 108 12.3 0.000002 Both
    263765_at At2g21540 SEC14 cytosolic factor, putative 135 64 2.1 0.000985 64 42 1.5 0.002042 Early
    249346_at At5g40780 Lys- and His-specific transporter, putative 783 170 4.6 0.002599 877 624 1.4 0.054790 Early
    264520_at At1g10010 Amino acid permease, putative 91 35 2.6 0.005939 167 34 4.8 0.000000 Late
    265002_at At1g24400 Lys- and His-specific transporter 156 41 3.8 0.024933 311 142 2.2 0.000005 Late
    259580_at At1g28030 Oxidoreductase, 2OG-Fe(II) oxygenase family protein 52 44 1.2 0.276557 95 32 3.0 0.000024 Late
    265064_at At1g61630 Equilibrative nucleoside transporter, putative (ENT7) 70 40 1.7 0.013220 67 29 2.3 0.000179 Late
    259844_at At1g73560 Protease inhibitor/seed storage/lipid transfer protein 49 22 2.2 0.005234 91 19 4.9 0.000122 Late
    264482_at At1g77210 Sugar transporter, putative 73 65 1.1 0.601658 118 55 2.1 0.000012 Late
    257366_s_at At2g03040 Transmembrane protein related 159 39 4.1 0.005632 184 22 8.3 0.000008 Late
    266276_at At2g29330 Tropinone reductase, putative 68 30 2.3 0.007410 68 28 2.4 0.000243 Late
    254453_at At4g21120 Amino acid permease family protein 211 64 3.3 0.007991 259 88 2.9 0.000000 Late
    246887_at At5g26250 Sugar transporter, putative 159 61 2.6 0.015475 146 37 4.0 0.000013 Late
    248275_at At5g53520 Oligopeptide transporter OPT family protein 43 32 1.3 0.204722 80 20 3.9 0.000002 Late
    248019_at At5g56480 Protease inhibitor/seed storage/lipid transfer protein 61 50 1.2 0.206709 111 38 2.9 0.000015 Late
Protein degradation
    256486_at At1g31450 Aspartyl protease family protein 263 18 14.8 0.001311 607 17 35.1 0.000000 Both
    245738_at At1g44130 Nucellin protein, putative 117 10 12.3 0.002988 94 8 11.2 0.000067 Both
    259368_at At1g69100 Aspartyl protease family protein 546 13 42.6 0.003756 952 33 29.1 0.000000 Both
    252499_s_at At3g46840 Subtilase family protein 389 34 11.6 0.000398 443 26 17.2 0.000001 Both
    245589_at At4g15040 Subtilase family protein 171 23 7.3 0.000154 66 17 4.0 0.000055 Both
    254336_at At4g22050 Aspartyl protease family protein 681 37 18.3 0.000096 714 67 10.6 0.000000 Both
    246684_at At5g33340 Aspartyl protease family protein 98 25 4.0 0.000228 189 21 9.1 0.000001 Both
    247798_at At5g58830 Subtilase family protein 73 15 5.0 0.002673 235 13 17.7 0.000000 Both
    254237_at At4g23520 Cys proteinase, putative 67 28 2.4 0.000243 41 22 1.9 0.000254 Early
    247697_at At5g59810 Subtilase family protein 92 43 2.1 0.002288 56 44 1.3 0.003872 Early
    264067_x_at At2g28010 Aspartyl protease family protein 33 19 1.7 0.039844 78 13 5.9 0.000004 Late
    250345_at At5g11940 Subtilase family protein 58 15 3.9 0.005591 154 14 11.4 0.000000 Late
Signal transduction
    263740_at At2g20660 Rapid alkalinization factor (RALF) family protein 82 33 2.4 0.000758 90 24 3.8 0.000000 Both
    245158_at At2g33130 RALF family protein 435 34 12.8 0.000989 437 38 11.4 0.000000 Both
    266418_at At2g38750 Annexin 4 (ANN4) 274 62 4.4 0.003825 220 67 3.3 0.000001 Both
    251514_at At3g59260 Pirin, putative 202 33 6.1 0.001924 171 30 5.7 0.000002 Both
    255489_at At4g02650 Epsin N-terminal homology domain-containing protein 448 22 20.7 0.004357 1,286 32 39.8 0.000000 Both
    245177_at At5g12380 Annexin, putative 190 17 11.2 0.000393 215 17 12.8 0.000000 Both
    249013_at At5g44700 Leu-rich repeat transmembrane protein kinase 81 31 2.6 0.001303 75 30 2.5 0.000305 Both
    261285_at At1g35720 Annexin 1 (ANN1) 168 122 1.4 0.273697 393 108 3.6 0.000012 Late
    257869_at At3g25160 ER lumen protein retaining receptor family protein 86 51 1.7 0.030431 147 52 2.8 0.000020 Late
    250090_at At5g17330 Glutamate decarboxylase 1 (GAD 1) 195 21 9.2 0.005864 222 25 9.0 0.000000 Late
Energy metabolism
    259268_at At3g01070 Plastocyanin-like domain-containing protein 79 28 2.8 0.002166 126 26 4.8 0.000001 Both
    253634_at At4g30590 Plastocyanin-like domain-containing protein 705 56 12.6 0.000057 500 41 12.3 0.000005 Both
    252897_at At4g39490 Cytochrome P450 family protein, At4g38480 62 19 3.4 0.004614 134 18 7.5 0.000000 Both
    248236_at At5g53870 Plastocyanin-like domain-containing protein 1,236 60 20.7 0.000394 653 165 4.0 0.000009 Both
    262528_at At1g17260 ATPase 10, plasma membrane type, putative 45 26 1.7 0.385722 344 102 3.4 0.000002 Late
    261396_at At1g79800 Plastocyanin-like domain-containing protein 49 40 1.2 0.236124 69 31 2.2 0.000064 Late
    266563_at At2g23990 Plastocyanin-like domain-containing protein 195 47 4.2 0.011017 953 41 23.3 0.000015 Late
    255690_at At4g00360 Cytochrome P450, putative 49 23 2.1 0.018309 79 29 2.7 0.000026 Late
    254489_at At4g20800 FAD-binding domain-containing protein 47 11 4.4 0.027803 121 11 11.3 0.000000 Late
Transcriptional regulation
    267528_at At2g45650 MADS-box protein (AGL6) 245 59 4.2 0.002904 359 170 2.1 0.000048 Both
    249338_at At5g41090 No apical meristem (NAM) family protein 166 18 9.1 0.000764 77 13 5.8 0.000000 Both
    260212_at At1g74480 RWP-RK domain-containing protein 55 36 1.5 0.071151 65 29 2.2 0.001510 Late
    266969_at At2g39540 Gibberellin-regulated family protein 41 38 1.1 0.593532 179 32 5.6 0.000003 Late
    254619_at At4g18770 Myb family transcription factor (MYB98) 109 59 1.8 0.063530 110 41 2.7 0.000002 Late
    251114_at At5g01380 Expressed protein 25 26 1.0 0.855505 62 23 2.7 0.000211 Late
    248240_at At5g53950 No apical meristem (NAM) family protein 224 201 1.1 0.399544 269 110 2.4 0.000013 Late
Secondary metabolism
    260386_at At1g74010 Strictosidine synthase family protein 110 21 5.1 0.001313 368 22 16.4 0.000010 Both
    264401_at At1g61720 Dihydroflavonol 4-reductase family (BAN) 54 45 1.2 0.693582 535 176 3.0 0.000017 Late
    260335_at At1g74000 Strictosidine synthase family protein 144 72 2.0 0.005947 104 39 2.7 0.000038 Late
    254283_s_at At4g22870 Leucoanthocyanidin dioxygenase, putative 81 85 1.0 0.880918 416 183 2.3 0.000118 Late
    249215_at At5g42800 Dihydroflavonol 4-reductase 31 26 1.2 0.546725 301 84 3.6 0.000030 Late
Plant development/organogenesis
    262113_at At1g02820 Late embryogenesis abundant 3 family protein 82 33 2.5 0.000057 76 27 2.8 0.000001 Both
    262659_at At1g14240 Nucleoside phosphatase family protein 80 25 3.3 0.000911 92 16 5.8 0.000000 Both
    262549_at At1g31290 PAZ domain-containing protein 140 99 1.4 0.096043 169 63 2.7 0.000011 Late
    252234_at At3g49780 Phytosulfokines 3 (PSK3) 17 9 2.0 0.386279 163 43 3.8 0.000014 Late
    251301_at At3g61880 Cytochrome P450, putative 94 51 1.9 0.003809 91 41 2.2 0.000032 Late
Protein biosynthesis/DNA catabolism
    245883_at At5g09500 40S ribosomal protein S15 (RPS15C) 85 18 4.7 0.002080 54 9 5.9 0.000028 Early
    260438_at At1g68290 Bifunctional nuclease, putative 44 28 1.6 0.093917 149 25 5.9 0.000104 Late
a

Affymetrix probe set number.

b

Arabidopsis Genome Initiative number.

c

Fold change for signal intensity of wild type/signal intensity of spl.

d

P values calculated by Student's t test for two-sample equal variance.

e

Expressed ovule stage.