Figure 8.

A, RT-qPCR expression profiles of UGT genes in wild-type plants and ugt mutants after Pst-AvrRpm1 challenge. Transcript levels were quantified for UGT73B3 and UGT73B5 in wild-type plants (black circles) and in ugt73b3 mutant and ugt73b5 mutant, respectively (white circles). Values correspond to the mean and sd of duplicates. B, Bacterial growth of Pst in Arabidopsis leaves of wild-type plants and ugt D mutants. Leaves were infiltrated with 10⁵ cfu mL⁻¹ Pst DC3000 or Pst-AvrRpm1 and harvested 24 and 72 h after inoculation. The bacterial population (log cfu cm⁻²) was quantified in wild-type plants (black bars), ugt73b3 (gray bars), and ugt73b5 (white bars) mutants. The graph represents the mean and sd of three replicates. In the incompatible interaction, the bacterial amount in mutant plants was significantly higher than that of the wild type at 72 h post inoculation, according to one-tailed Student's t test (P value = 0.03 and 0.0004 for ugt73b3 and ugt73b5, respectively). Experiment was repeated with similar results.