Table V.
Primers used in this study
At-F and At-R were used to amplify RT-PCR products specific for each gene. PPE-gene-site primers were used to assay the editing extent of a specific site by PPE (Peeters and Hanson, 2002).
| Gene | Primers |
|---|---|
| ccb206a | Atccb206F: ATGAGACGACTTTTTCTTGAACTATAT |
| Atccb206R: TTAATCTTGTAAACTAATCGAGACC | |
| PPE-ccb206-C24: TTTTATTAGGGAGCCTGGTCTTGACTC | |
| PPE-ccb206-28: GATTGGTGTGGAGGAGAAGATCAG | |
| ccb452 | Atccb452F: GGTCCAACTACATAACTTTTTCTT |
| Atccb452R: ATTATGAACTCCACGGAACTTTCT | |
| PPE-ccb452-155: TCGCTGACCTATCGCGTGCTAAAAAG | |
| PPE-ccb452-415: CCTTGAATGTAAATAGACCAAAAGAGAG | |
| orfxa | AtOrfxF: CACTTTTAGCTTTGAATTACTTAT |
| AtOrfxR: ATTGATAGTTACTTTGCCAGGTTC | |
| PPE-orfx-409: CCAAACATTGGGAACGACCCAGG | |
| rps4a | Atrps4F: TCCCCATTAAGATTTCAAACTTGTCGTC |
| Atrps4R: TTATATGTTTTGGCCACGTCCGTTTCTG | |
| PPE-rps4-1057: Atrps4-R | |
| ccb203a | Atccb203F: TGGACACGGGGAGGGAGCAG |
| Atccb203R: CATAACATAACGGGGCGGGGTTGC | |
| PPE-ccb203-320: CAGCATGGAAAAGTCACAATATTAAG | |
| nad4 | Atnad4F: AAGTGGTCTTATTCTGTGTCC |
| Atnad4R: TTTGCCATGTTGCACTAAGTTACT | |
| PPE-nad4-896: CAATGATCTTCTTTAGATCGATCTG | |
| PPE-nad4-1405: GCAGTCCGGGAACACTTTGGGGTG | |
| nad5 | Atnad5F: GAAGGAAGCGCTATAATGACCACT |
| Atnad5R: CGATCGATTATCTACCCAAGAAGA | |
| PPE-nad5-1916: GCAATGTTACTTGGTTCAACTCTATTTGTGACC | |
| nad7 | Atnad7F: ACTAGGAAAAGGCAAATCAAAAAT |
| Atnad7R: ATCCACCTCTCCAAACACAATA | |
| PPE-nad7-200: GAGTACAAAACTTATCTTCAAGCTTTACC |
a
Transcripts of these genes do not contain introns; a control without reverse transcriptase was performed to check for DNA contamination.