Figure 6.

Expression analysis of wheat lipocalins in response to diurnal cycles. Plants were germinated for 8 d at 20°C under a 16-h-day/8-h-night photoperiod. Beginning on day 8 at 8:00, plants were grown at 20°C for 12 h (from 08:00–20:00) then kept at 20°C for 52 h (A, controls) or transferred at 4°C for 52 h (B, cold acclimated). Leaf samples were harvested at each time point, and RNA was extracted and analyzed as described in Figure 3. Gray areas represent the dark periods.