Table 1.

Estimates of input RNA quantities and resulting yield for 10 000 cells (reference) and 1000 and 250 cells (test), respectively. Calculation of the average fold yield of aRNA after two rounds of amplification are based on the assumption that 2% of total RNA represents mRNA.

Cell sample size	~Total RNA	~mRNA (2% of total RNA)	Synthetic mRNA	Sum mRNA	Average aRNA yield	Average amplification factor
10 000	~115 ng	~2.3 ng	3.0 ng	5.3 ng	54 μg	1.02 × 104
1000	~11.5 ng	~0.23 ng	0.3 ng	0.53 ng	3.66 μg	0.69 × 104
250	~2.88 ng	~0.058 ng	0.075 ng	0.133 ng	1.18 μg	0.89 × 104