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. 2026 Apr 10;17:1816023. doi: 10.3389/fmicb.2026.1816023

FIGURE 2.

Panel figure displays six bar charts (A–F) depicting halo diameter measurements (centimeters) for six strains under varying conditions, and two grids of petri dish photographs (G, H) comparing visual halo formation for each strain under the same conditions. Each chart and photograph set compares control, pH6.0, pH8.0, 0.2% and 0.8% glucose, and 28°C treatments, with strains labeled along the y-axis and conditions along the x-axis. Statistically significant differences are annotated in the bar graphs.

Effect of pH, glucose concentration and temperature on in vitro antagonistic activity of Gram-positive and Gram-negative antagonists against E. amylovora. Control conditions consisted of growth on AB with 0.4% glucose, pH 7.0 at room temperature (22°C). Variations of pH, glucose concentration and incubation temperature were explored in representative (A–C) Gram-positive (blue) and (D–F) Gram-negative (yellow) isolates. Asterisks denote statistically significant differences between the indicated variable and control conditions: *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; ns, non-significant (P > 0.05). (G,H) Representative images of the assay with Gram-positives (G) and Gram-negatives (H). Priestia sp. ABR6, Bacillus sp. 2175, Bacillus sp. BWC, Rahnella sp. EL51, Pseudomonas sp. ABR5, Pseudomonas sp. 2180.