TABLE 1.
Microorganisms co-isolated with E. amylovora from symptomatic apple tissues: source, identity and phenotypes.
| Activity halo diameter (mm ± SD)bc | |||||||
|---|---|---|---|---|---|---|---|
| Isolate ID | Sample/host | Location | Identificationa | Amylase | Protease | Lipase | % SU ± SD |
| Gram-positive | |||||||
| 2173 | Necrosed leaf/“Gala” apple tree | Kennewick, WA | Microbacterium sp. | 2.8 ± 0.6 cd | UA e | UA d | 43.1 ± 0.5 ce |
| ABR10 | Necrosed leaf/“Envy” apple tree | Othello, WA | Microbacterium sp. | UA e | 6.3 ± 0.6 b | UA d | 28.7 ± 0.2 g |
| ABR6 | Necrosed leaf/“Cripps Pink” apple tree | Benton City, WA | Priestia sp. | 3.2 ± 0.3 c | 9.3 ± 0.6 a | 5.8 ± 0.3 c | 44.6 ± 2.0 bc |
| ABR8 | Rootstock canker/M9 | Sunnyside, WA | Curtobacterium sp. | 1.7 ± 0.6 d | 2.3 ± 0.6 cd | UA d | 45.9 ± 0.8 bc |
| 2175 | Necrosed leaf/“Gala” apple tree | Kennewick, WA | Bacillus sp. | 3.8 ± 0.3 bc | 7.5 ± 1.5 b | 11.0 ± 1.0 a | 41.0 ± 1.2 de |
| BWC | Necrosed shoot/“Gala” apple tree | Kennewick, WA | Bacillus sp. | 11.0 ± 1.0 a | 3.7 ± 0.6 c | 10.0 ± 0.0 ab | 56.0 ± 1.4 a |
| Gram-negative | |||||||
| EL51 | Necrosed fruit/“Gala” apple tree | Kennewick, WA | Rahnella sp. | UA e | 1.7 ± 0.6 de | UA d | 36.1 ± 0.4 f |
| 2186 | Necrosed shoot/“Gala” apple tree | Kennewick, WA | Erwinia sp. | 4.7 ± 0.6 b | UA e | UA d | 47.5 ± 1.0 b |
| ABR1b | Symptomatic apple tree | WA | Pantoea sp. | UA e | UA e | UA d | 40.6 ± 2.1 e |
| ABR1s | Symptomatic apple tree | WA | Pantoea sp. | UA e | UA e | UA d | 28.6 ± 0.6 g |
| ABR5 | Necrosed leaf/“Gala” apple tree | Kennewick, WA | Pseudomonas sp. | UA e | 10.0 ± 0.5 a | UA d | 44.2 ± 2.0 bcd |
| 2180 | Necrosed leaf/“Jazz” apple tree | Kennewick, WA | Pseudomonas sp. | UA e | 9.7 ± 0.6 a | 9.0 ± 2.7 ab | 46.1 ± 0.9 bc |
| Yeast | |||||||
| 2176 | Necrosed leaf/“Gala” apple tree | Kennewick, WA | Rhodotorula sp. | UA e | UA e | 10.00 ± 1.0 ab | 26.9 ± 0.4 g |
aIdentifications based on BLASTn analysis against NCBI refseq_rna database. For some isolates, high alignment scores among congeneric species were observed. For consistency, identifications are presented at the genus level.
bAmylase, protease and lipase activities quantified as halo diameter minus colony diameter. Siderophore production is quantified as percentage of Siderophore Units (% SU) by the liquid CAS assay. Data represents mean ± standard deviation (SD) (n = 3). UA, undetected (0 mm).
cIn each column, different letters indicate statistically significant differences between the isolates (α = 0.05).