Skip to main content
. 2005 Nov;15(11):1477–1486. doi: 10.1101/gr.4212705

Figure 6.

Figure 6.

Physical characterization of the SPANX-B genomic TAR isolates. (A) Chromosomal-size DNA was isolated from yeast transformants containing a SPANX-B locus, digested with NotI, CHEF gel electrophoresis-separated, and blot-hybridized with an exon 2 probe. The SPANX-B locus was analyzed in 14 patients and 18 controls; the figure illustrates only 10 randomly chosen individuals. Lanes 1–5 correspond to four unaffected controls (C1, C2, C3, C4 and C3); lanes 6–10 correspond to five patients (236–005, 029–049, 032–003, 075–014 and 236–005). For control C3 and patient 236–005, two independent TAR isolates were included into the analysis to exclude internal deletions during isolation. The leading bands from 60 kb to 90 kb correspond to the linearized molecules of SPANX-B, correspondingly. (B) Alu profile characterization of TAR YACs containing SPANX-B. Total yeast DNA was isolated from the transformants and digested to completion with TaqI. Fragments were separated by gel electrophoresis, transferred to a nylon membrane, and hybridized with an Alu probe.