Fig. 6. Saccharomyces cerevisiae importin α enters the nucleus in an importin β-independent manner. (A) Wild-type yeast cells were transformed with importin α–GFP, ΔIBB importin α–GFP or ΔIBB ED importin α–GFP. GFP fusion proteins were visualized by direct fluorescence microscopy. Corresponding differential interference contrast (DIC) images are shown. (B) Myc-tagged importin α proteins were detected by indirect immunofluorescence using an anti-myc antibody. Cells were also stained with DAPI to show the position of the nucleus. Corresponding DIC images are shown.