Fig. 1. Experiments with continuous exposure to light.

A Power spectrum of P. tricornutum (WT) Ratio obtained with laser diffractometry experiments (see Fig. S1 for the original time series). The dotted line represents the measured data, and the solid line corresponds to the Gaussian fit, as detailed in the Supplementary Materials. Colors indicate the wavelength with which cells were illuminated (430, 530, 617, 660, and 780 nm). Light irradiance was kept constant for all wavelengths (8 µmol photons m⁻² s⁻¹). B DPH deactivation/activation (green/blue areas) spectrum (Δ Abs) for the WT resulting from absorption differences between the synthesized active state of DPH and the inactive conformation (adapted from Duchêne et al.¹⁸). C Peak amplitude (Pk) of the power spectrum (shown in A) normalized by the full width at half maximum (FWHM) of the power spectrum for the wavelength of the light used (arbitrary units). Solid circles correspond to experiments with wild-type (WT) and transformed, but DPH non-mutated strains, and empty triangles correspond to experiments carried out with DPH knockout strains (KO). Data are shown as mean ± SD (n = 3 independent biological replicates).