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. Author manuscript; available in PMC: 2026 Apr 25.
Published before final editing as: Dev Cell. 2026 Apr 21:S1534-5807(26)00122-X. doi: 10.1016/j.devcel.2026.03.010

Table 1.

Reagents for CUBIC-based whole mount muscle clarification

Solution Reagent Concentration
CUBIC-1 Urea (Sigma, #51456) 25%
80% (wt/wt) Quadrol (Sigma, #122262) 25%
Triton-X100 1%
Distilled water 49%
CUBIC-2 Urea (Sigma, #51456) 25%
Triethanolamine (TEA) (Sigma, #90278) 25%
Sucrose (Sigma, #S0389) 15%
Distilled water 35%