Figure 2.

RT + IAV increases the presence of Arg-1 positive cells, localized to regions of lung pathology. Lungs were collected from mice 26 week following exposure to RT and IAV, either alone or in combination, where early IAV was administered 1 week after RT, and late IAV was administered 20 weeks after RT, or from non-exposed control mice. Immunohistological staining was conducted for A) the pan-macrophage marker CD64, and B) arginase-1 (Arg-1). C). Quantification of Arg-1 positive cells within regions of pathology. D). Quantification of Arg-1 positive cells in regions without pathology. Bars represent mean ± SEM. Symbols (circles) represent each mouse. n=3–5 mice per condition. Significantly different comparisons are as indicated by brackets. *P<0.05, **P<0.01, ***P<0.001. ROI’s were acquired at 100x.