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. Author manuscript; available in PMC: 2026 Apr 25.
Published in final edited form as: Am J Respir Cell Mol Biol. 2026 Jan 1;74(1):94–105. doi: 10.1165/rcmb.2025-0113OC

Figure 4.

Figure 4.

RT and IAV acutely alter lung macrophage population size and phenotype. CD45+ immune cells isolated from lung digests, collected 1 week following exposure to RT + IAV (or 1 week post IAV alone, or 2 weeks post RT alone) were analyzed by flow cytometry. A) Macrophages were identified by the exclusion of doublets, dead cells, and Ly6G+ cells, and the positive expression of CD45, F4/80, and, for TR-AMs, autofluorescence. Distinct lung macrophage subsets were identified as tissue-resident alveolar macrophages (TR-AMs; CD11blow, CD11c+), monocyte-derived alveolar macrophages (Mo-AMs; CD11b+, CD11c+), and monocyte-derived macrophages (Mo-MPs; CD11b+, CD11c-). B) Proportions of TR-AMs, Mo-AMs, and Mo-MPs within the total macrophage population. C) Percentages of TR-AMs, Mo-AMs, and Mo-MPs in each experimental condition. D) Percentages of CD206+ cells within TR-AMs Mo-AMs, and Mo-MPs. E) Percentages of Ly6C+ cells within Mo-AMs and Mo-MPs. Bars represent mean ± SEM. Symbols (circles) represent each mouse. n=4 mice per condition. Significantly different comparisons are as indicated by brackets. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001.