Figure 5.

RT and IAV alter the composition of macrophage populations in the lung. CD45+ cells were isolated from lung digests collected 1 week following exposure to RT + IAV (or 1 week post IAV alone, or 2 weeks post RT alone) and analyzed by single cell sequencing. Immune cell identification was performed, and populations identified as monocytes/macrophages were subclustered for further analysis. A) Seven unique subsets were identified. B) Expression of macrophage and monocyte markers within each subset. C) Coexpression of Cd68, a macrophage marker, with Ccr2, a monocyte marker, varied across the multiple subsets. D) Monocyte and macrophage marker expression corresponds with monocyte and macrophage designation, respectively, with mixed expression in the M1 Monocyte/Macrophage subset. E) Expression of markers of monocyte trafficking and differentiation do not overlap with markers of resident alveolar macrophages. F) The size and composition of the monocyte and macrophage subsets present varied between each condition. G) The proportion of cells in M1 Monocyte/Macrophage 0 or 1 subsets varied between IAV and RT + IAV. H) Gene coexpression of Itgax (CD11c) with Itgam (CD11b) in monocyte and macrophage clusters