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Fig. 6. Creation of second-strand L1 cDNA. (A) The full L1 transposition reaction requires the utilization of two nicks in the target DNA. Arrows indicate the position of the primers (JB1180 and 2NP) used for PCR. (B) Five prime end insertion. Lane 1, wild-type ORF2 protein; lane 2, EN mutant ORF2p; lane 3, RT mutant ORF2p. (C) Black arrowheads indicate the position of target DNA–L1 5′ cDNA junctions.
