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. 2005 Dec 9;33(22):7011–7018. doi: 10.1093/nar/gki1007

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© The Author 2005. Published by Oxford University Press. All rights reserved

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Activity of E.coli IF3 and IF3_mt in proofreading the initiation complex. The abilities of E.coli IF3 (circles) and IF3_mt (squares) to promote the dissociation of a pre-formed complex [E.coli 30S:poly(U):[¹⁴C]AcPhe-tRNA] (open symbols) were measured in the presence of various concentrations of IF3 as described in Materials and Methods. The value for 100% complex remaining is 2.1 pmol. The effects of E.coli IF3 and IF3_mt on a pre-formed complex [30S:poly(A,U,G):[³⁵S]fMet-tRNA] (closed symbols) were tested under similar conditions. The 100% value for these experiments was 0.14 pmol.