Figure 2.

Colony PCR analysis of bacterial clones obtained from H1, H3, H4 and H5 genomic DNA as in Figure 1B. Ligation products were used to transform E.coli. Analytical colony PCR was with the oligonucleotides in Figure 1B. Examples of clones representing both alleles in H5 and H3 were obtained as shown. In contrast, no full-length H1 or H4 clones were recovered. Markers, ‘M’ are as in Figure 1C. For H3, 4 and 5, a single gel image has been cut with lanes removed. The H1 image is from a different gel. To simplify cross-comparisons here, as well as in Figures 3A and 4, images from different gels have been size-adjusted in the vertical dimension.