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. 2005 Dec 9;33(22):e186. doi: 10.1093/nar/gni189

Figure 5.

Figure 5

ABI fragment analysis of PCR templated by sibling H8 and H4 genomic DNA (top and middle) and the yeast clone H4#4 (bottom). Peaks occurring in 2 bp increments are PCR stutter products. For each sample, the main peak migrates at an apparent size of 315 bp. The coincidence of the H8 and H4 peaks demonstrates the reproducibility of both PCR amplification as well as capillary gel electrophoresis. The main peak from the H4#4 PCR products is at the same position as the PCR products from H4 genomic DNA, identifying H4#4 as a true clone of the genomic sequence. The H4#4 fragment is known by sequence to be 323 bp thus the H4 (and H8) allele migrates as though it were 8 bp shorter than its true size.