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. 2005 Dec 7;33(21):6795–6804. doi: 10.1093/nar/gki982

Figure 3.

Figure 3

MPP6 predominantly co-sediments with nuclear exosome complexes at 60S–80S in glycerol gradients. Cytoplasmic (A) and nuclear (B) extracts from HEp-2 cells were fractionated in a 5–40% (v/v) glycerol gradient. The sedimentation of hRrp4, MPP6 and PM/Scl-100 was determined by immunoblotting. The sedimentation of the large rRNAs was determined by agarose gel electrophoresis and ethidium bromide staining and used as markers (40S and 60S) in the gradient. U1 snRNA was used as marker for 12S complexes.