Figure 6.
The TH enhancing activity of the pesticide acetochlor revealed in the brains of germinally transgenic premetamorphic tadpoles. Abbreviations: a, anterior; NS, not significant; p, posterior. (A) NF stage 50–52 germinally transgenic tadpoles bearing a TH/bZIP-eGFP transgene were pretreated for 24 hr with 10−12 M T3 and then rinsed and fed for 24 hr.Fluorescence was measured in tadpole brains after 48 hr exposure to 10−10 M T3 or to 10−10 M T3 plus 10−8 M acetochlor. Values shown are mean ± SE of three experiments expressed as multiples of induction, where 1 = control expression in the absence of T3. Data were normalized and analyzed by Student’s t-test. (B) Representative photographs of strongly fluorescent tadpoles brain from each group (n = 15 tadpoles per group). Bars = 0.4 mm.
*p < 0.05. #p < 0.001.