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. 2005 Dec 6;33(21):6942–6951. doi: 10.1093/nar/gki998

Figure 4.

Figure 4

Steady-state levels of PecoRV•CR and PecoRV•M transcripts in the presence or in the absence of C.EcoRV. RNA was purified from the E.coli HB101 cells harboring the indicated plasmids and primer extension reactions were performed to reveal 5′ end of divergent RNAs arising from the EcoRV regulatory region. The R primer allows the detection of rightward transcripts from pR or pM (PecoRV•CR or PecoRV•M transcripts, correspondingly). The L primer allows the detection of leftward transcripts from pR or pM (PecoRV•M or PecoRV•CR transcripts, correspondingly). The sequencing reactions' marker lanes were prepared using the pM or pR plasmids and primers used for primer extension.