Figure 7: Tumor progression trees inferred by ScisTree on scRNA data from a preclinical study of anti-CTLA-4 immunotherapy for melanoma.

a. Tumor progression trees inferred by ScisTree from smartseq-control data (“Control-tree”, left) and smartseq-treated data (“Treated-tree”, right). The clonal trees are labeled by the number of mutations that were inferred to have been acquired for the first time in the evolution of the tumor for each node/subclone. Based on shared mutations labeled at the root of each subtree, there are three major clades (B3, B4, and B5) that were previously identified in (14) and are colored accordingly. b. An optimal alignment between Control- and Treated-tree, “Alignment-tree”, as determined by computing omltd. The clonal tree is labeled by the number of common mutations with concordant placement between Control- and Treated-trees.