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. 2005 Dec 15;116(1):90–100. doi: 10.1172/JCI26169

Figure 4.

Figure 4

The role of IL-12 produced by SOCS1-silenced DCs in breaking self tolerance and inducing antitumor immunity. (A and B) Inability to inhibit preestablished B16 tumor by IL-12–deficient SOCS1-siRNA DCs. WT or IL-12p35–KO mice were inoculated s.c. with B16 tumor cells (2.5 × 105) and 3 days later were immunized with 1.5 × 106 TRP2-pulsed, transduced WT, or IL-12p35–KO DCs with ex vivo LPS maturation. Tumor growth (A) and percent survival (B) (n = 6 mice/group) curves represent 1 of 2 independent experiments. P < 0.01, GFP-siRNA DCs compared with SOCS1-siRNA DCs. (C) CD8+ T cell responses induced by IL-12–deficient SOCS1-siRNA DCs. CD8+ T cells isolated from the pooled splenocytes of immunized WT or IL-12p35–KO mice were subjected to IFN-γ ELISPOT assays. An irrelevant peptide (OVA-I peptide) was used as a negative control. *P < 0.01 versus SOCS1-siRNA DCs.