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. 2005 Dec 15;116(1):90–100. doi: 10.1172/JCI26169

Figure 6.

Figure 6

WT DCs constitutively expressing IL-12 were insufficient to overcome self tolerance at the host level. (A) Inability to inhibit preestablished B16 tumors by Ad–IL-12 DCs. BM DCs were transduced with Ad–IL-12 (MOIs of 300 or 1,000) or LV-SOCS-siRNA or LV-GFP-siRNA (MOI of 5). WT mice were inoculated s.c. with B16 tumor cells (2.5 × 105) and 3 days later were immunized with 1.5 × 106 TRP2-pulsed DCs with ex vivo TNF-α maturation (50 ng/ml). After transfer of DCs, IL-12 (1 μg/mouse) was administered i.p. 3 times on days 1, 3, and 5. Tumor growth curves (n = 6 mice/group) represent 1 of 3 independent experiments. (B and C). Antigen-specific CD8+ T cell responses. Splenocytes or CD8+ T cells isolated from the pooled splenocytes of immunized mice were subjected to IFN-γ ELISPOT assays (B) or intracellular IFN-γ staining (C). *P < 0.01 versus SOCS1-siRNA DCs plus IL-12.