Table 1.

Effect of A23187 on apoptosis. The B lymphoblasts were cultured in basal (X-VIVO15) medium in the absence or presence of 200 nM A23187 for 48 hours. Percentages of FITC-annexinV positive cells were determined as described in Methods. Two Scott B lymphoblasts independently immortalized from the patient's lymphocytes, one daughter's and two control B lymphoblasts from unrelated individuals were used for these studies. Each cell line was analyzed twice. Results are means ± SD from the four experiments for Scott and control B lymphoblasts respectively and the two experiments for daughter's cells.

	% of annexin V positive cells
	untreated	+ A23187
Control	18.5 ± 10.7	33.7 ± 17.6
Daughter	14.9 ± 8.6	29.6 ± 0.3
Scott	48.7 ± 7.6	64.7 ± 12.9